Laboratory of Biomolecular cryo-EM

Visualizing the Molecules of Life by cryo-EM

Research in the Reichow Lab is inspired by the molecular mechanisms driving biology. We apply cutting-edge methods in the high-resolution imaging technology of cryo-electron microscopy (cryo-EM), together with biochemical, biophysical and computational tools to unveil the inner-workings of individual protein molecules, nature’s nano-machines. It is our hope that the atomic-level models and mechanistic insights developed by our research will help to elucidate the biomolecular basis of human health and disease. 

The Reichow Lab is located at Oregon Health & Science University, within the Department of Chemical Physiology and Biochemistry and the Vollum Institute.

Publications

Find links to our published work and preprints available on BioRxiv.

Learn more about how we are applying cryo-EM to visualize biomolecular complexes and contribute to understanding the molecular basis of disease.

Research Projects

The Reichow Lab is always interested in discussing opportunities to join our research team. Follow the link below for descriptions and contact information.

Join Our Team

Selected Publications

  • Jarodsky et al. BioRxiv 2025

    Jarodsky JM, Myers JB, Reichow SL*, Reversible lipid mediated pH-gating of connexin-46/50 by cryo-EM, BioRxiv (2025) Feb

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  • Flores & O'Neill et al. BioRxiv 2025

    Flores JA†, O’Neill SE†, Jarodsky JM, Reichow SL*, Calcium induced N-terminal gating and pore collapse in connexin-46/50 gap junctions, BioRxiv (2025) Feb

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  • Miller et al. BioRxiv 2024

    Miller AP and Reichow SL*, Mechanism of small heat shock protein client sequestration and induced polydispersity. BioRxiv (2024) Dec

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  • McFarland et al. Nat Comm 2024

    McFarland R, Noroozi R, Miller AP, Reichow SL*, Dynamic fibrillar assembly of αB-crystallin induced by perturbation of the conserved NT-IXI motif resolved by cryo-EM. Nature Communications (2024) Nov 28;15:10336 [BioRxiv 2024 Mar]

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  • Miller et al. J Mol Bio 2024

    Miller AP, O’Neill SE, Lampi KJ, Reichow SL*, The α-crystallin chaperones undergo a quasi-ordered co-aggregation process in response to saturating client interaction. Journal of Molecular Biology (2024) Apr 15;436(8):168499 [BioRxiv (2023) Aug]

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  • Mostofian & McFarland et al. J Mol Bio 2022

    Mostofian B†, McFarland R†, Estelle A, Howe J, Barbar E*, Reichow SL*, Zuckerman DM*, Continuum dynamics and statistical correction of compositional heterogeneity in multivalent IDP oligomers resolved by single-particle EM. Journal of Molecular Biology (2022) May 15;434(9):167520 [BioRxiv (2021) May]

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  • Buonarati & Miller et al. Cell Reports 2021

    Buonarati OR†, Miller AP†, Coultrap SJ, Bayer KU* and Reichow SL*, Conserved and divergent features of neuronal CaMKII holoenzyme structure, function and high-order assembly. Cell Reports (2021) Dec 28;37(13):110168 DOI [Front Cover] [BioRxiv (2021) Jan]

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  • Tong & Khan et al. Biophys J 2021

    Tong JJ†, Khan U†, Haddad BG, Minogue BS, Beyer EC, Berthoud VM, Reichow SL*, Ebihara L*, Molecular mechanisms underlying enhanced hemichannel function of a cataract-associated Cx50 mutant. Biophysical Journal (2021) Dec 21;120(24):5644-5656 [BioRxiv (2021) Aug]

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  • Yue & Haddad et al. J Physiol 2021

    Yue B†, Haddad BG†, Khan U, Chen H, Atalla M, Zhang Z, Zuckerman DM, Reichow SL* and Bai D*, Connexin 46 and coonexin 50 gap junction channel properties are shaped by structural and dynamic features of their N-terminal domains. Journal of Physiology (2021) Jul 1;599(13):3313-3335 [BioRxiv (2020) Jun]

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  • Flores. Haddad & Dolan et al. Nat Comm 2020

    Flores JA†, Haddad BG†, Dolan KA†, Myers JB, Yoshioka CC, Copperman J, Zuckerman DM, Reichow SL*, Connexin-46/50 in a dynamic lipid environment resolved by CryoEM at 1.9 Å. Nature Communications (2020) Aug 28;11:4331 [BioRxiv (2020) April]

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  • Myers & Haddad et al. Nature 2018

    Myers JB†, Haddad BG†, O’Neill SE, Chorev DS, Yoshioka CC, Robinson CV, Zuckerman DM and Reichow SL*, Structure of native lens connexin-46/50 intercellular channels by CryoEM. Nature (2018) Dec 12;564(7736):372-377

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  • Clark et al. eLife 2018

    Clark SA, Myers JB, Fiala R, Novacek J, Pearce G, King A, Heierhorst J, Reichow SL, Barbar E, Multivalency Regulates Activity in an Intrinsically Disordered Transcription Factor. eLife (2018) May 1;7:e36258

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  • Myers & Zaegel et al. Nat Comm 2017

    Myers JB†, Zaegel V†, Coultrap SJ, Miller AP, Bayer KU*, Reichow SL*, The CaMKII holoenzyme structure in activation-competent conformations. Nature Communications (2017) Jun 7;8:15742

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